Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (AP)

Primary AntibodyLSBio Guarantee

Antibody:	Goat Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Invertebrate (Other)
Format:	Liquid

See all Aequorea victoria GFP antibodies â†’

$887.00each

Catalog Number: LS-C154196-1

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Overview

Description: GFP antibody LS-C154196 is an AP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Invrt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GFP
Recommended Dilution: ELISA: 1:2000 - 1:8000
Recommended Dilution: IF/ICC: 1:200 - 1:1000
Recommended Dilution: IHC: 1:200 - 1:1000
Recommended Dilution: WB: 1:500 - 1:2500
UniProt Number: P42212
SwissProt: P42212

Target

Target: Aequorea victoria GFP
Antigen Species: Invertebrate (Other)
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Antibody

Host Species: Goat
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Alkaline Phosphatase
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified