Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Biotin Mouse Anti-GFP Antibody - Western Blot. Western Blot showing detection of Green Fluorescent Protein. 50 ng of GFP (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated - 600-306-215 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 28 kD molecular weight position expected for GFP. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Monoclonal Mouse anti-Aequorea victoria GFP Antibody (clone 9F9.F9, Biotin, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Invertebrate (Other) |
| Format: | Lyophilized |
$966.00each
Catalog Number: LS-C154212-1
Catalog Number: LS-C154212
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- GFP antibody LS-C154212 is a biotin-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Invrt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- GFP
- Recommended Dilution: ELISA
- 1:50000 - 1:200000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P42212
- SwissProt
- P42212
Target
- Target
- Aequorea victoria GFP
- Antigen Species
- Invertebrate (Other)
- Epitope
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein by both Western blot and ELISA. No reaction is seen against RFP.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 9F9.F9
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
