Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate. This antibody detects a 27 kD band corresponding to the epitope tag GFP. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4C with the primary antibody diluted in 5% BLOTTO to 1:1000, followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
GFP Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Invertebrate (Other) |
| Format: | Liquid |
$475.00each
Catalog Number: LS-C154219-100
Catalog Number: LS-C154219
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- GFP antibody LS-C154219 is an unconjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 7 publications.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Invrt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GFP
- Recommended Dilution: ELISA
- 1:20000 - 1:120000
- Recommended Dilution: IF/ICC
- 1:500 - 1:5000
- Recommended Dilution: IHC
- 1:200 - 1:3000
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P42212
- SwissProt
- P42212
Target
- Target
- Aequorea victoria GFP
- Antigen Species
- Invertebrate (Other)
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.29 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. Papadopoulos C, Arato K, Lilienthal E, Zerweck J, Schutkowski M, Chatain N, Mller-Newen G, Becker W, de la Luna S. The Journal of biological chemistry. 2011;286:5494-505.
PubMed: 21127067 · PMC: PMC3037663
The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. Torres J, Funk HM, Zegers MM, ter Beest MB. The Journal of biological chemistry. 2011;286:10834-46.
PubMed: 21278252 · PMC: PMC3060534
Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. Schmidt FI, Bleck CK, Helenius A, Mercer J. The EMBO journal. 2011;30:3647-61.
PubMed: 21792173 · PMC: PMC3181475
Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A. Cell death and differentiation. 2012;19:1152-61.
PubMed: 22322860 · PMC: PMC3374078
High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Jin H, Zangar RC. Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]. 2012;Unit 17.15.
PubMed: 22511115
Hepatocellular Carcinoma Cell-Secreted Exosomal MicroRNA-210 Promotes Angiogenesis In Vitro and In Vivo. Lin XJ, Fang JH, Yang XJ, Zhang C, Yuan Y, Zheng L, Zhuang SM. Molecular therapy. Nucleic acids. 2018 June;11:243-252.
PubMed: 29858059 · PMC: PMC5992447
Fibrotic microenvironment promotes the metastatic seeding of tumor cells via activating the fibronectin 1/secreted phosphoprotein 1-integrin signaling. Zhang C, Wu M, Zhang L, Shang LR, Fang JH, Zhuang SM. Oncotarget. 2016 July;7:45702-45714.
PubMed: 27329720 · PMC: PMC5216754
