CAST / Calpastatin Antibody
Catalog Number: LS-C155216-100
100 µL (0.7 mg/mL) / $459.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81287-20
Catalog Number: LS-G81287
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_173060) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Origin Species
- Human
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPTETKAVK TEPEKKSQST KLSVVHEKKS QEGKPKEHTE PKSLPKQASD TGSNDAHNKK61AVSRSAEQQP SEKSTEPKTK PQDMISAGGE SVAGITAISG KPGDKKKEKK SLTPAVPVES121KPDKPSGKSG MDAALDDLID TLGGPEETEE ENTTYTGPEV SDPMSSTYIE ELGKREVTIP181PKYRELLAKK EGITGPPADS SKPIGPDDAI DALSSDFTCG SPTAAGKKTE KEESTEVLKA241QSAGTVRSAA PPQEKKRKVE KDTMSDQALE ALSASLGTRQ AEPELDLRSI KEVDEAKAKE301EKLEKCGEDD ETIPSEYRLK PATDKDGKPL LPEPEEKPKP RSESELIDEL SEDFDRSECK361EKPSKPTEKT EESKAAAPAP VSEAVCRTSM CSIQSAPPEP ATLKGTVPDD AVEALADSLG421KKEADPEDGK PVMDKVKEKA KEEDREKLGE KEETIPPDYR LEEVKDKDGK PLLPKESKEQ481LPPMSEDFLL DALSEDFSGP QNASSLKFED AKLAAAISEV VSQTPASTTQ AGAPPRDTSQ541SDKDLDDALD KLSDSLGQRQ PDPDENKPME DKVKEKAKAE HRDKLGERDD TIPPEYRHLL601DDNGQDKPVK PPTKKSEDSK KPADDQDPID ALSGDLDSCP STTETSQNTA KDKCKKAASS661SKAPKNGGKA KDSAKTTEET SKPKDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
686 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
