Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119515-100
Catalog Number: LS-G119515
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_001042444), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Origin Species
- Human
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPTETKAVK TEPEKKSQST KPKSLPKQAS DTGSNDAHNK KAVSRSAEQQ PSEKSTEPKT61KPQDMISAGG ESVAGITAIS GKPGDKKKEK KSLTPAVPVE SKPDKPSGKS GMDAALDDLI121DTLGGPEETE EENTTYTGPE VSDPMSSTYI EELGKREVTI PPKYRELLAK KEGITGPPAD181SSKPIGPDDA IDALSSDFTC GSPTAAGKKT EKEESTEVLK AQSAGTVRSA APPQEKKRKV241EKDTMSDQAL EALSASLGTR QAEPELDLRS IKEVDEAKAK EEKLEKCGED DETIPSEYRL301KPATDKDGKP LLPEPEEKPK PRSESELIDE LSEDFDRSEC KEKPSKPTEK TEESKAAAPA361PVSEAVCRTS MCSIQSAPPE PATLKGTVPD DAVEALADSL GKKEADPEDG KPVMDKVKEK421AKEEDREKLG EKEETIPPDY RLEEVKDKDG KPLLPKESKE QLPPMSEDFL LDALSEDFSG481PQNASSLKFE DAKLAAAISE VVSQTPASTT QAGAPPRDTS QSDKDLDDAL DKLSDSLGQR541QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV KPPTKKSEDS601KKPADDQDPI DALSGDLDSC PSTTETSQNT AKDKCKKAAS SSKAPKNGGK AKDSAKTTEE661TSKPKDDTRT RPLEQKLISE EDLAANDILD YKDDDDKV
698 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.