Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99547-100
Catalog Number: LS-G99547
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_001750), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQPGQKPAA SPRPRRAAAA RRTHEHVSEK TSESPSKPGE KKGSDEKKAA SLGSSQSSRT61YAGGTASATK VSASSGATSK SSSMNPTETK AIPVSQQMEG PHLPNKKKHK KQAVKTEPEK121KSQSTKLSVV HEKKSQEGKP KEHTEPKSLP KQASDTGSND AHNKKAVSRS AEQQPSEKST181EPKTKPQDMI SAGGESVAGI TAISGKPGDK KKEKKSLTPA VPVESKPDKP SGKSGMDAAL241DDLIDTLGGP EETEEENTTY TGPEVSDPMS STYIEELGKR EVTIPPKYRE LLAKKEGITG301PPADSSKPIG PDDAIDALSS DFTCGSPTAA GKKTEKEEST EVLKAQSSGT VRSAAPPQEK361KRKVEKDTMS DQALEALSAS LGTRQAEPEL DLRSIKEVDE AKAKEEKLEK CGEDDETIPS421EYRLKPATDK DGKPLLPEPE EKPKPRSESE LIDELSEDFD RSECKEKPSK PTEKTEESKA481AAPAPVSEAV CRTSMCSIQS APPEPATLKG TVPDDAVEAL ADSLGKKEAD PEDGKPVMDK541VKEKAKEEDR EKLGEKEETI PPDYRLEEVK DKDGKPLLPK ESKEQLPPMS EDFLLDALSE601DFSGPQNASS LKFEDAKLAA AISEVVSQTP ASTTQAGAPP RDTSQSDKDL DDALDKLSDS661LGQRQPDPDE NKPMEDKVKE KAKAEHRDKL GERDDTIPPE YRHLLDDNGQ DKPVKPPTKK721SEDSKKPADD QDPIDALSGD LDSCPSTTET SQNTAKDKCK KAASSSKAPK NGGKAKDSAK781TTEETSKPKD DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
791 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.