Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105396-100
Catalog Number: LS-G105396
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_173060), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Origin Species
- Human
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPTETKAVK TEPEKKSQST KLSVVHEKKS QEGKPKEHTE PKSLPKQASD TGSNDAHNKK61AVSRSAEQQP SEKSTEPKTK PQDMISAGGE SVAGITAISG KPGDKKKEKK SLTPAVPVES121KPDKPSGKSG MDAALDDLID TLGGPEETEE ENTTYTGPEV SDPMSSTYIE ELGKREVTIP181PKYRELLAKK EGITGPPADS SKPIGPDDAI DALSSDFTCG SPTAAGKKTE KEESTEVLKA241QSAGTVRSAA PPQEKKRKVE KDTMSDQALE ALSASLGTRQ AEPELDLRSI KEVDEAKAKE301EKLEKCGEDD ETIPSEYRLK PATDKDGKPL LPEPEEKPKP RSESELIDEL SEDFDRSECK361EKPSKPTEKT EESKAAAPAP VSEAVCRTSM CSIQSAPPEP ATLKGTVPDD AVEALADSLG421KKEADPEDGK PVMDKVKEKA KEEDREKLGE KEETIPPDYR LEEVKDKDGK PLLPKESKEQ481LPPMSEDFLL DALSEDFSGP QNASSLKFED AKLAAAISEV VSQTPASTTQ AGAPPRDTSQ541SDKDLDDALD KLSDSLGQRQ PDPDENKPME DKVKEKAKAE HRDKLGERDD TIPPEYRHLL601DDNGQDKPVK PPTKKSEDSK KPADDQDPID ALSGDLDSCP STTETSQNTA KDKCKKAASS661SKAPKNGGKA KDSAKTTEET SKPKDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
686 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.