CAST / Calpastatin Antibody
Catalog Number: LS-C155216-100
100 µL (0.7 mg/mL) / $459.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G81284-20
Catalog Number: LS-G81284
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_001190442) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Origin Species
- Human
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH61TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI121SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP181EVSDPMSSTY IEELGKREVT IPPKYRELLA KPIGPDDAID ALSSDFTCGS PTAAGKKTEK241EESTEVLKAQ SAGTVRSAAP PQEKKRKVEK DTMSDQALEA LSASLGTRQA EPELDLRSIK301EVDEAKAKEE KLEKCGEDDE TIPSEYRLKP ATDKDGKPLL PEPEEKPKPR SESELIDELS361EDFDRSECKE KPSKPTEKTE ESKAAAPAPV SEAVCRTSMC SIQSAPPEPA TLKGTVPDDA421VEALADSLGK KEADPEDGKP VMDKVKEKAK EEDREKLGEK EETIPPDYRL EEVKDKDGKP481LLPKESKEQL PPMSEDFLLD ALSEDFSGPQ NASSLKFEDA KLAAAISEVV SQTPASTTQA541GAPPRDTSQS DKDLDDALDK LSDSLGQRQP DPDENKPMED KVKEKAKAEH RDKLGERDDT601IPPEYRHLLD DNGQDKPVKP PTKKSEDSKK PADDQDPIDA LSGDLDSCPS TTETSQNTAK661DKCKKAASSS KAPKNGGKAK DSAKTTEETS KPKDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
695 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
