LSBio
PathPlus™ ITGAM / CD11b Antibody (clone M1/70.15)

Anti-CD11b antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ ITGAM / CD11b Antibody (clone M1/70.15)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Human, Mouse, Rabbit
Format:Liquid
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$470.00each
Catalog Number: LS-B3760-0.05

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Overview

Description
CD11b antibody LS-B3760 is an unconjugated rat monoclonal antibody to CD11b (ITGAM) from mouse. It is reactive with human, mouse and rabbit. Validated for Flow, IF, IHC and IP.
Application
FC, IF, IHC, IHC-F, IHC-P, IP
Reactivity
Hu, Ms, Rb
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Antigen CD11b (p170) | CD11B | CD11b antigen | CR3A | Integrin alpha-M | MAC-1 | MO1A | Neutrophil adherence receptor | MAC1A | SLEB6 | CR-3 alpha chain | ITGAM
Recommended Dilution: FC
1:50
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P11215
SwissProt
P11215

Target

Target
ITGAM / CD11b
Antigen Species
Mouse
Specificity
Mouse, human, rabbit ITGAM
Epitope
Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Gene Name
ITGAM

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
M1/70.15
Origin Species
Human

Immunogen

Immunogen
T cell enriched splenocytes from B10 mice

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.