Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N-Term antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
ACTB / Beta Actin Antibody (N-Terminus, clone 3H2.E4.E12)
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| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Pig, Rabbit, Rat |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C153787-100
Catalog Number: LS-C153787
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- Description
- Beta Actin antibody LS-C153787 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Por, Rb, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- UniProt Number
- P60709
- NCBI GenBank
- NM_001101 | NP_001092.1
- SwissProt
- P60709
Target
- Target
- Human ACTB / Beta Actin
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
- Gene Name
- ACTB
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 3H2.E4.E12
- Isotype
- IgG2a
- Light Chain
- Lambda Light Chain
Immunogen
- Immunogen
- Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with β-Actin peptide near the N-Terminus followed by hybridoma development.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl