LSBio
IHC-plus™ ACTB / Beta Actin Antibody (clone 4C2)

Anti-Beta Actin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ ACTB / Beta Actin Antibody (clone 4C2)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Chicken, Human, Mouse, Pig, Rabbit, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B7160-0.05

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Overview

Description
Beta Actin antibody LS-B7160 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, FC, ICC, IHC, IHC-F, IHC-P, WB
Reactivity
Ck, Hu, Ms, Por, Rb, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Recommended Dilution: IHC-F
1:100 - 1:500
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
P60709
SwissProt
P60709

Target

Target
ACTB / Beta Actin
Antigen Species
Human
Specificity
The epitope is highly conserved and is therefore expected to react with many other species. The antibody does not cross react with other actin isoforms.
Epitope
The epitope is highly conserved and is therefore expected to react with many other species. The antibody does not cross react with other actin isoforms.
Gene Name
ACTB

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
4C2
Origin Species
Human

Immunogen

Immunogen
Peptide comprising the N-Terminus nonapeptide of beta-cytoplasmic actin with an acetylated N-Terminus (Ac-DDDIAALVV-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: 4°C Long term: Aliquot and store at -20°C.
Recommended Storage Buffer
PBS

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