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IHC-plus™ Monoclonal Mouse anti-Human ACTB / Beta Actin Antibody (clone 4C2, N-Terminus, IHC, IF, WB)

Human Prostate: Formalin Fixed, Paraffin Embedded (FFPE)

IHC-plus™ Monoclonal Mouse anti-Human ACTB / Beta Actin Antibody (clone 4C2, N-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all ACTB / Beta Actin antibodies →
$383.00each
Catalog Number: LS-B11002-0.05

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Overview

Description
Beta Actin antibody LS-B11002 is an unconjugated mouse monoclonal antibody to human Beta Actin (ACTB) (N-Terminus). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
IF, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Recommended Dilution: IHC-F
1:50 - 1:100
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P60709
SwissProt
P60709

Target

Target
ACTB / Beta Actin
Antigen Species
Human
Specificity
Mouse anti Human actin beta, clone 4C2, recognizes cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kD cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. Clone 4C2 is highly specific for cytoplasmic actin beta and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following Sendai virus infection and the specific role of the viral M protein in actin remodeling.
Epitope
Recognizes cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kD cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. Clone 4C2 is highly specific for cytoplasmic actin beta and does not react with other actin isoforms. Has been used to look at the remodeling of the actin cytoskeleton following Sendai virus infection and the specific role of the viral M protein in actin remodeling.
Gene Name
ACTB

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
4C2
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide corresponding to the acetylated N-Terminus nonapeptide of beta-cytoplasmic actin, coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.