LSBio
IHC-plus™ ACTB / Beta Actin Antibody (aa2-16)

Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ ACTB / Beta Actin Antibody (aa2-16)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B6503-50

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Overview

Description
Beta Actin antibody LS-B6503 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa2-16) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Predicted Reactivity
Bovine, Dog, Chicken, Guinea Pig, Hamster, Horse, Arthropod (Insect / Arachnid / Crustacean), Mammal (Other), Non-Human Primate, Pig, Rabbit, Sheep, Xenopus
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
0.25 - 1 µg/ml
UniProt Number
P60709
SwissProt
P60709

Target

Target
ACTB / Beta Actin
Antigen Species
Human
Specificity
Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen.
Epitope
The NCBI accession number for actin is AAA51567. This can not be used to test heart or muscle lysate loading.
Gene Name
ACTB

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Amino acids 2-15 (CDDDIAALVIDNGSG) of human beta actin were used as the immunogen. Percent identity by BLAST analysis: Seq squirt, Sea anemone (100%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (93%).

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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