LSBio
IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus)

Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B1625-50

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Overview

Description
Beta Actin antibody LS-B1625 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 20 publications.
Application
ICC, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Recommended Dilution: IHC-P
1:100
UniProt Number
P60709
SwissProt
P60709

Target

Target
ACTB / Beta Actin
Antigen Species
Mouse
Specificity
Peptide-KLH, NH2 terminus
Epitope
Peptide-KLH, NH2 terminus
Gene Name
ACTB

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from mouse ACTB / Beta-actin.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Concentration
0.15 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
Phosphate-buffered

Gap-junctional communication is required for mitotic clonal expansion during adipogenesis. Yanagiya T, Tanabe A, Hotta K. Obesity (Silver Spring, Md.). 2007;15:572-82.

PubMed: 17372306


IL-21 induces inhibitor of differentiation 2 and leads to complete abrogation of anaphylaxis in mice. Kishida T, Hiromura Y, Shin-Ya M, Asada H, Kuriyama H, Sugai M, Shimizu A, Yokota Y, Hama T, Imanishi J, Hisa Y, Mazda O. Journal of immunology (Baltimore, Md. : 1950). 2007;179:8554-61.

PubMed: 18056403


Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Ouimet M, Wang MD, Cadotte N, Ho K, Marcel YL. Arteriosclerosis, thrombosis, and vascular biology. 2008;28:1144-50.

PubMed: 18369155


Activated protein C up-regulates IL-10 and inhibits tissue factor in blood monocytes. Toltl LJ, Beaudin S, Liaw PC. Journal of immunology (Baltimore, Md. : 1950). 2008;181:2165-73.

PubMed: 18641355


Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. Sawada T, Nishiyama C, Kishi T, Sasazuki T, Komazawa-Sakon S, Xue X, Piao JH, Ogata H, Nakayama J, Taki T, Hayashi Y, Watanabe M, Yagita H, Okumura K, Nakano H. The Journal of biological chemistry. 2008;283:26820-8.

PubMed: 18667423 · PMC: PMC3258922


Knockdown of macrophage migration inhibitory factor disrupts adipogenesis in 3T3-L1 cells. Ikeda D, Sakaue S, Kamigaki M, Ohira H, Itoh N, Ohtsuka Y, Tsujino I, Nishimura M. Endocrinology. 2008;149:6037-42.

PubMed: 18703634


Muramyldipeptide augments the actions of lipopolysaccharide in mice by stimulating macrophages to produce pro-IL-1 and by down-regulation of the suppressor of cytokine signaling 1 (SOCS1). Shikama Y, Kuroishi T, Nagai Y, Iwakura Y, Shimauchi H, Takada H, Sugawara S, Endo Y. Innate immunity. 2011;17:3-15.

PubMed: 19897531


The pathogen recognition receptor NOD2 regulates human FOXP3+ T cell survival. Rahman MK, Midtling EH, Svingen PA, Xiong Y, Bell MP, Tung J, Smyrk T, Egan LJ, Faubion WA. Journal of immunology (Baltimore, Md. : 1950). 2010;184:7247-56.

PubMed: 20483763


Genetic deletion of the P2Y2 receptor offers significant resistance to development of lithium-induced polyuria accompanied by alterations in PGE2 signaling. Zhang Y, Pop IL, Carlson NG, Kishore BK. American journal of physiology. Renal physiology. 2012;302:F70-7.

PubMed: 21975874


c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Piao X, Komazawa-Sakon S, Nishina T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, Van Rooijen N, Schtz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H. Science signaling. 2012;5:ra93.

PubMed: 23250397


Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. Sun M, Liu C, Zhao N, Meng K, Zhang Z. Experimental and therapeutic medicine. 2018 June;15:5215-5220.

PubMed: 29904405 · PMC: PMC5996703


URG11 promotes proliferation and induced apoptosis of LNCaP cells. Sun C, Zhang G, Cheng S, Qian H, Li D, Liu M. International journal of molecular medicine. 2019 May;43:2075-2085.

PubMed: 30864678 · PMC: PMC6443344


Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells. Zhang Y, Yang J, Zhou X, Wang N, Li Z, Zhou Y, Feng J, Shen D, Zhao W. International journal of molecular medicine. 2019 October;44:1357-1365.

PubMed: 31432092 · PMC: PMC6713428


Knockdown of KLK12 inhibits viability and induces apoptosis in human colorectal cancer HT-29 cell line. Li Q, Zhou X, Fang Z, Zhou H. International journal of molecular medicine. 2019 November;44:1667-1676.

PubMed: 31485623 · PMC: PMC6777684


miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1. Zhu L, Yang S, Wang J. International journal of molecular medicine. 2019 November;44:1824-1832.

PubMed: 31485607 · PMC: PMC6777686


Effect of estradiol on high glucose-induced osteoblast injury. Li G, Jiang X, Liu L, Liu X, Liu H, Zhang Z. Molecular medicine reports. 2019 October;20:3019-3026.

PubMed: 31432111 · PMC: PMC6755179


GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Canjun Zhao #, Jin Zhang #, Litian Ma #, Hao Wu, Hui Zhang, Jialin Su, Bizu Geng, Qinghua Yao, Jin Zheng. OncoTargets and therapy. 2020 July;13:6265-6277.

PubMed: 32636646 · PMC: PMC7335312


Recombinant human growth hormone treatment of mice suppresses inflammation and apoptosis caused by skin flap ischemia-reperfusion injury. Ben Liu, Qingjia Xu, Juntao Wang, Junhao Lin, Yantao Pei, Yidong Cui, Gang Wang, Lei Zhu. Journal of cellular biochemistry. 2019 October;120:18162-18171.

PubMed: 31144385


Up-regulation of microRNA-574 attenuates lipopolysaccharide- or cecal ligation and puncture-induced sepsis associated with acute lung injury. Wenwen Sun, Hong Li, Jin Gu. Cell biochemistry and function. 2020 October;38:847-858.

PubMed: 32090367


The function of long noncoding RNA HOTAIRM1 in the progression of prostate cancer cells. Lei Wang, Longning Wang, Qingfen Wang, Bahman Yosefi, Sen Wei, Xiaodong Wang, Daqing Shen. Andrologia. 2021 March;53:e13897.

PubMed: 33368390

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