ACTB / Beta Actin Antibody
Catalog Number: LS-C137559-100
100 µL (1 mg/mL) / $454.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ ACTB / Beta Actin Antibody (aa350-375)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Bovine, Guinea Pig, Goat, Hamster, Human, Mouse, Non-Human Primate, Pig, Rat, Sheep |
| Format: | Liquid |
$383.00each
Catalog Number: LS-B323-50
Catalog Number: LS-B323
For bulk orders or custom formulations:
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- Description
- Beta Actin antibody LS-B323 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa350-375) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Bov, GP, Gt, Ham, Hu, Ms, NHP, Por, Rt, Sh
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Actin, cytoplasmic 1 | ACTB | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: IF/ICC
- 1:500 - 1:2000
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 1:1000 - 1:4000
- UniProt Number
- P60709
- NCBI GenBank
- NM_001101 | NP_001092.1
- SwissProt
- P60709
Target
- Target
- ACTB / Beta Actin
- Antigen Species
- Human
- Specificity
- Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
- Epitope
- Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
- Gene Name
- ACTB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.9 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
