ARHGEF1 Antibody
Catalog Number: LS-C404555
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G80089-20
Catalog Number: LS-G80089
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF1 (NM_199002) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 115-kD protein | Arp2/3 complex 34 kd subunit | ARHGEF1 | GEF1 | LBCL2 | p115RhoGEF | LSC | Lsc homolog | SUB1.5 | p115-RHOGEF
- Origin Species
- Human
- NCBI GenBank
- NM_004706
- SwissProt
- Q92888
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASLSTWSSP AEPREMEDFA RGAASPGPSR PGLVPVSIIG AEDEDFENEL ETNSEEQNSQ61FQSLEQVKRR PAHLMALLQH VALQFEPGPL LCCLHADMLG SLGPKEAKKA FLDFYHSFLE121KTAVLRVPVP PNVAFELDRT RADLISEDVQ RRFVQEVVQS QQVAVGRQLE DFRSKRLMGM181TPWEQELAQL EAWVGRDRAS YEARERHVAE RLLMHLEEMQ HTISTDEEKS AAVVNAIGLY241MRHLGVRTKS GDKKSGRNFF RKKVMGNRRS DEPAKTKKGL SSILDAARWN RGEPQVPDFR301HLKAEVDAEK PGATDRKGGV GMPSRDRNIG APGQDTPGVS LHPLSLDSPD REPGADAPLE361LGDSSPQGPM SLESLAPPES TDEGAETESP EPGDEGEPGR SGLELEPEEP PGWRELVPPD421TLHSLPKSQV KRQEVISELL VTEAAHVRML RVLHDLFFQP MAECLFFPLE ELQNIFPSLD481ELIEVHSLFL DRLMKRRQES GYLIEEIGDV LLARFDGAEG SWFQKISSRF CSRQSFALEQ541LKAKQRKDPR FCAFVQEAES RPRCRRLQLK DMIPTEMQRL TKYPLLLQSI GQNTEEPTER601EKVELAAECC REILHHVNQA VRDMEDLLRL KDYQRRLDLS HLRQSSDPML SEFKNLDITK661KKLVHEGPLT WRVTKDKAVE VHVLLLDDLL LLLQRQDERL LLKSHSRTLT PTPDGKTMLR721PVLRLTSAMT REVATDHKAF YVLFTWDQEA QIYELVAQTV SERKNWCALI TETAGSLKVP781APASRPKPRP SPSSTREPLL SSSENGNGGR ETSPADARTE RILSDLLPFC RPGPEGQLAA841TALRKVLSLK QLLFPAEEDN GAGPPRDGDG VPGGGPLSPA RTQEIQENLL SLEETMKQLE901ELEEEFCRLR PLLSQLGGNS VPQPGCTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
927 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
