ARHGEF1 Antibody
Catalog Number: LS-C404555
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80088-20
Catalog Number: LS-G80088
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF1 (NM_198977) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 115-kD protein | Arp2/3 complex 34 kd subunit | ARHGEF1 | GEF1 | LBCL2 | p115RhoGEF | LSC | Lsc homolog | SUB1.5 | p115-RHOGEF
- Origin Species
- Human
- NCBI GenBank
- NM_004706
- SwissProt
- Q92888
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM61ALLQHVALQF EPGPLVLRVP VPPNVAFELD RTRADLISED VQRRFVQEVV QSQQVAVGRQ121LEDFRSKRLM GMTPWEQELA QLEAWVGRDR ASYEARERHV AERLLMHLEE MQHTISTDEE181KSAAVVNAIG LYMRHLGVRT KSGDKKSGRN FFRKKVMGNR RSDEPAKTKK GLSSILDAAR241WNRGEPQVPD FRHLKAEVDA EKPGATDRKG GVGMPSRDRN IGAPGQDTPG VSLHPLSLDS301PDREPGADAP LELGDSSPQG PMSLESLAPP ESTDEGAETE SPEPGDEGEP GRSGLELEPE361EPPGWRELVP PDTLHSLPKS QVKRQEVISE LLVTEAAHVR MLRVLHDLFF QPMAECLFFP421LEELQNIFPS LDELIEVHSL FLDRLMKRRQ ESGYLIEEIG DVLLARFDGA EGSWFQKISS481RFCSRQSFAL EQLKAKQRKD PRFCAFVQEA ESRPRCRRLQ LKDMIPTEMQ RLTKYPLLLQ541SIGQNTEEPT EREKVELAAE CCREILHHVN QAVRDMEDLL RLKDYQRRLD LSHLRQSSDP601MLSEFKNLDI TKKKLVHEGP LTWRVTKDKA VEVHVLLLDD LLLLLQRQDE RLLLKSHSRT661LTPTPDGKTM LRPVLRLTSA MTREVATDHK AFYVLFTWDQ EAQIYELVAQ TVSERKNWCA721LITETAGSLK VPAPASRPKP RPSPSSTREP LLSSSENGNG GRETSPADAR TERILSDLLP781FCRPGPEGQL AATALRKVLS LKQLLFPAEE DNGAGPPRDG DGVPGGGPLS PARTQEIQEN841LLSLEETMKQ LEELEEEFCR LRPLLSQLGG NSVPQPGCTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
879 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
