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ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF1 antibodies →
$504.00each
Catalog Number: LS-G103469-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF1 (NM_198977), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
115-kD protein | Arp2/3 complex 34 kd subunit | ARHGEF1 | GEF1 | LBCL2 | p115RhoGEF | LSC | Lsc homolog | SUB1.5 | p115-RHOGEF
Origin Species
Human
NCBI GenBank
NM_004706
SwissProt
Q92888

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM
61ALLQHVALQF EPGPLVLRVP VPPNVAFELD RTRADLISED VQRRFVQEVV QSQQVAVGRQ
121LEDFRSKRLM GMTPWEQELA QLEAWVGRDR ASYEARERHV AERLLMHLEE MQHTISTDEE
181KSAAVVNAIG LYMRHLGVRT KSGDKKSGRN FFRKKVMGNR RSDEPAKTKK GLSSILDAAR
241WNRGEPQVPD FRHLKAEVDA EKPGATDRKG GVGMPSRDRN IGAPGQDTPG VSLHPLSLDS
301PDREPGADAP LELGDSSPQG PMSLESLAPP ESTDEGAETE SPEPGDEGEP GRSGLELEPE
361EPPGWRELVP PDTLHSLPKS QVKRQEVISE LLVTEAAHVR MLRVLHDLFF QPMAECLFFP
421LEELQNIFPS LDELIEVHSL FLDRLMKRRQ ESGYLIEEIG DVLLARFDGA EGSWFQKISS
481RFCSRQSFAL EQLKAKQRKD PRFCAFVQEA ESRPRCRRLQ LKDMIPTEMQ RLTKYPLLLQ
541SIGQNTEEPT EREKVELAAE CCREILHHVN QAVRDMEDLL RLKDYQRRLD LSHLRQSSDP
601MLSEFKNLDI TKKKLVHEGP LTWRVTKDKA VEVHVLLLDD LLLLLQRQDE RLLLKSHSRT
661LTPTPDGKTM LRPVLRLTSA MTREVATDHK AFYVLFTWDQ EAQIYELVAQ TVSERKNWCA
721LITETAGSLK VPAPASRPKP RPSPSSTREP LLSSSENGNG GRETSPADAR TERILSDLLP
781FCRPGPEGQL AATALRKVLS LKQLLFPAEE DNGAGPPRDG DGVPGGGPLS PARTQEIQEN
841LLSLEETMKQ LEELEEEFCR LRPLLSQLGG NSVPQPGCT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

879 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.