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ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF1 antibodies →
$725.00each
Catalog Number: LS-G103484-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF1 (NM_199002), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
115-kD protein | Arp2/3 complex 34 kd subunit | ARHGEF1 | GEF1 | LBCL2 | p115RhoGEF | LSC | Lsc homolog | SUB1.5 | p115-RHOGEF
Origin Species
Human
NCBI GenBank
NM_004706
SwissProt
Q92888

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASLSTWSSP AEPREMEDFA RGAASPGPSR PGLVPVSIIG AEDEDFENEL ETNSEEQNSQ
61FQSLEQVKRR PAHLMALLQH VALQFEPGPL LCCLHADMLG SLGPKEAKKA FLDFYHSFLE
121KTAVLRVPVP PNVAFELDRT RADLISEDVQ RRFVQEVVQS QQVAVGRQLE DFRSKRLMGM
181TPWEQELAQL EAWVGRDRAS YEARERHVAE RLLMHLEEMQ HTISTDEEKS AAVVNAIGLY
241MRHLGVRTKS GDKKSGRNFF RKKVMGNRRS DEPAKTKKGL SSILDAARWN RGEPQVPDFR
301HLKAEVDAEK PGATDRKGGV GMPSRDRNIG APGQDTPGVS LHPLSLDSPD REPGADAPLE
361LGDSSPQGPM SLESLAPPES TDEGAETESP EPGDEGEPGR SGLELEPEEP PGWRELVPPD
421TLHSLPKSQV KRQEVISELL VTEAAHVRML RVLHDLFFQP MAECLFFPLE ELQNIFPSLD
481ELIEVHSLFL DRLMKRRQES GYLIEEIGDV LLARFDGAEG SWFQKISSRF CSRQSFALEQ
541LKAKQRKDPR FCAFVQEAES RPRCRRLQLK DMIPTEMQRL TKYPLLLQSI GQNTEEPTER
601EKVELAAECC REILHHVNQA VRDMEDLLRL KDYQRRLDLS HLRQSSDPML SEFKNLDITK
661KKLVHEGPLT WRVTKDKAVE VHVLLLDDLL LLLQRQDERL LLKSHSRTLT PTPDGKTMLR
721PVLRLTSAMT REVATDHKAF YVLFTWDQEA QIYELVAQTV SERKNWCALI TETAGSLKVP
781APASRPKPRP SPSSTREPLL SSSENGNGGR ETSPADARTE RILSDLLPFC RPGPEGQLAA
841TALRKVLSLK QLLFPAEEDN GAGPPRDGDG VPGGGPLSPA RTQEIQENLL SLEETMKQLE
901ELEEEFCRLR PLLSQLGGNS VPQPGCT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

927 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.