Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTT / Huntingtin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118183-100
Catalog Number: LS-G118183
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Huntingtin(HTT) (NM_002111), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Huntingtin | Huntington disease protein | IT15 | HD | HTT | HD protein
- Origin Species
- Human
- NCBI GenBank
- NM_002111
- SwissProt
- P42858
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 347.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATLEKLMKA FESLKSFQQQ QQQQQQQQQQ QQQQQQQQQQ PPPPPPPPPP PQLPQPPPQA61QPLLPQPQPP PPPPPPPPGP AVAEEPLHRP KKELSATKKD RVNHCLTICE NIVAQSVRNS121PEFQKLLGIA MELFLLCSDD AESDVRMVAD ECLNKVIKAL MDSNLPRLQL ELYKEIKKNG181APRSLRAALW RFAELAHLVR PQKCRPYLVN LLPCLTRTSK RPEESVQETL AAAVPKIMAS241FGNFANDNEI KVLLKAFIAN LKSSSPTIRR TAAGSAVSIC QHSRRTQYFY SWLLNVLLGL301LVPVEDEHST LLILGVLLTL RYLVPLLQQQ VKDTSLKGSF GVTRKEMEVS PSAEQLVQVY361ELTLHHTQHQ DHNVVTGALE LLQQLFRTPP PELLQTLTAV GGIGQLTAAK EESGGRSRSG421SIVELIAGGG SSCSPVLSRK QKGKVLLGEE EALEDDSESR SDVSSSALTA SVKDEISGEL481AASSGVSTPG SAGHDIITEQ PRSQHTLQAD SVDLASCDLT SSATDGDEED ILSHSSSQVS541AVPSDPAMDL NDGTQASSPI SDSSQTTTEG PDSAVTPSDS SEIVLDGTDN QYLGLQIGQP601QDEDEEATGI LPDEASEAFR NSSMALQQAH LLKNMSHCRQ PSDSSVDKFV LRDEATEPGD661QENKPCRIKG DIGQSTDDDS APLVHCVRLL SASFLLTGGK NVLVPDRDVR VSVKALALSC721VGAAVALHPE SFFSKLYKVP LDTTEYPEEQ YVSDILNYID HGDPQVRGAT AILCGTLICS781ILSRSRFHVG DWMGTIRTLT GNTFSLADCI PLLRKTLKDE SSVTCKLACT AVRNCVMSLC841SSSYSELGLQ LIIDVLTLRN SSYWLVRTEL LETLAEIDFR LVSFLEAKAE NLHRGAHHYT901GLLKLQERVL NNVVIHLLGD EDPRVRHVAA ASLIRLVPKL FYKCDQGQAD PVVAVARDQS961SVYLKLLMHE TQPPSHFSVS TITRIYRGYN LLPSITDVTM ENNLSRVIAA VSHELITSTT1021RALTFGCCEA LCLLSTAFPV CIWSLGWHCG VPPLSASDES RKSCTVGMAT MILTLLSSAW1081FPLDLSAHQD ALILAGNLLA ASAPKSLRSS WASEEEANPA ATKQEEVWPA LGDRALVPMV1141EQLFSHLLKV INICAHVLDD VAPGPAIKAA LPSLTNPPSL SPIRRKGKEK EPGEQASVPL1201SPKKGSEASA ASRQSDTSGP VTTSKSSSLG SFYHLPSYLK LHDVLKATHA NYKVTLDLQN1261STEKFGGFLR SALDVLSQIL ELATLQDIGK CVEEILGYLK SCFSREPMMA TVCVQQLLKT1321LFGTNLASQF DGLSSNPSKS QGRAQRLGSS SVRPGLYHYC FMAPYTHFTQ ALADASLRNM1381VQAEQENDTS GWFDVLQKVS TQLKTNLTSV TKNRADKNAI HNHIRLFEPL VIKALKQYTT1441TTCVQLQKQV LDLLAQLVQL RVNYCLLDSD QVFIGFVLKQ FEYIEVGQFR ESEAIIPNIF1501FFLVLLSYER YHSKQIIGIP KIIQLCDGIM ASGRKAVTHA IPALQPIVHD LFVLRGTNKA1561DAGKELETQK EVVVSMLLRL IQYHQVLEMF ILVLQQCHKE NEDKWKRLSR QIADIILPML1621AKQQMHIDSH EALGVLNTLF EILAPSSLRP VDMLLRSMFV TPNTMASVST VQLWISGILA1681ILRVLISQST EDIVLSRIQE LSFSPYLISC TVINRLRDGD STSTLEEHSE GKQIKNLPEE1741TFSRFLLQLV GILLEDIVTK QLKVEMSEQQ HTFYCQELGT LLMCLIHIFK SGMFRRITAA1801ATRLFRSDGC GGSFYTLDSL NLRARSMITT HPALVLLWCQ ILLLVNHTDY RWWAEVQQTP1861KRHSLSSTKL LSPQMSGEEE DSDLAAKLGM CNREIVRRGA LILFCDYVCQ NLHDSEHLTW1921LIVNHIQDLI SLSHEPPVQD FISAVHRNSA ASGLFIQAIQ SRCENLSTPT MLKKTLQCLE1981GIHLSQSGAV LTLYVDRLLC TPFRVLARMV DILACRRVEM LLAANLQSSM AQLPMEELNR2041IQEYLQSSGL AQRHQRLYSL LDRFRLSTMQ DSLSPSPPVS SHPLDGDGHV SLETVSPDKD2101WYVHLVKSQC WTRSDSALLE GAELVNRIPA EDMNAFMMNS EFNLSLLAPC LSLGMSEISG2161GQKSALFEAA REVTLARVSG TVQQLPAVHH VFQPELPAEP AAYWSKLNDL FGDAALYQSL2221PTLARALAQY LVVVSKLPSH LHLPPEKEKD IVKFVVATLE ALSWHLIHEQ IPLSLDLQAG2281LDCCCLALQL PGLWSVVSST EFVTHACSLI YCVHFILEAV AVQPGEQLLS PERRTNTPKA2341ISEEEEEVDP NTQNPKYITA ACEMVAEMVE SLQSVLALGH KRNSGVPAFL TPLLRNIIIS2401LARLPLVNSY TRVPPLVWKL GWSPKPGGDF GTAFPEIPVE FLQEKEVFKE FIYRINTLGW2461TSRTQFEETW ATLLGVLVTQ PLVMEQEESP PEEDTERTQI NVLAVQAITS LVLSAMTVPV2521AGNPAVSCLE QQPRNKPLKA LDTRFGRKLS IIRGIVEQEI QAMVSKRENI ATHHLYQAWD2581PVPSLSPATT GALISHEKLL LQINPERELG SMSYKLGQVS IHSVWLGNSI TPLREEEWDE2641EEEEEADAPA PSSPPTSPVN SRKHRAGVDI HSCSQFLLEL YSRWILPSSS ARRTPAILIS2701EVVRSLLVVS DLFTERNQFE LMYVTLTELR RVHPSEDEIL AQYLVPATCK AAAVLGMDKA2761VAEPVSRLLE STLRSSHLPS RVGALHGVLY VLECDLLDDT AKQLIPVISD YLLSNLKGIA2821HCVNIHSQQH VLVMCATAFY LIENYPLDVG PEFSASIIQM CGVMLSGSEE STPSIIYHCA2881LRGLERLLLS EQLSRLDAES LVKLSVDRVN VHSPHRAMAA LGLMLTCMYT GKEKVSPGRT2941SDPNPAAPDS ESVIVAMERV SVLFDRIRKG FPCEARVVAR ILPQFLDDFF PPQDIMNKVI3001GEFLSNQQPY PQFMATVVYK VFQTLHSTGQ SSMVRDWVML SLSNFTQRAP VAMATWSLSC3061FFVSASTSPW VAAILPHVIS RMGKLEQVDV NLFCLVATDF YRHQIEEELD RRAFQSVLEV3121VAAPGSPYHR LLTCLRNVHK VTTCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
3144 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.