HTT / Huntingtin Antibody
Catalog Number: LS-C822680-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-HTT / Huntingtin antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
IHC-plus™ HTT / Huntingtin Antibody (phospho-Ser421)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B36-50
Catalog Number: LS-B36
For bulk orders or custom formulations:
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- Description
- Huntingtin antibody LS-B36 is an unconjugated rabbit polyclonal antibody to human Huntingtin (HTT) (pSer421). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Huntingtin | Huntington disease protein | IT15 | HD | HTT | HD protein
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: IHC-P
- 10 - 20 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- P42858
- NCBI GenBank
- NM_002111 | NP_002102.4
- SwissProt
- P42858
Target
- Target
- HTT / Huntingtin
- Antigen Species
- Human
- Specificity
- This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Epitope
- This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Gene Name
- HTT
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Huntingtin pS421 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 400-425 of Human Huntington Disease Protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
