Western Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1µg/mL for overnight at 4°C. Secondary antibody: IRDye800® rabbit secondary antibody at 1:10,000 for 45 min at RT.
HTT / Huntingtin Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209358-100
Catalog Number: LS-C209358
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- Description
- Huntingtin antibody LS-C209358 is an unconjugated rabbit polyclonal antibody to Huntingtin (HTT) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Huntingtin | Huntington disease protein | IT15 | HD | HTT | HD protein
- Recommended Dilution: ELISA
- 1:20000 - 1:60000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:100 - 1:500
- UniProt Number
- P42858
- NCBI GenBank
- NM_002111 | NP_002102.4
- SwissProt
- P42858
Target
- Target
- Human HTT / Huntingtin
- Antigen Species
- Human
- Epitope
- This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
- Gene Name
- HTT
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 50% Glycerol
- Concentration
- 1.1 mg/mL
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl