LSBio
IHC-plus™ Polyclonal Rabbit anti-Human HTT / Huntingtin Antibody (C-Terminus, IHC, IF, WB)

Anti-HTT / Huntingtin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human HTT / Huntingtin Antibody (C-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$526.00each
Catalog Number: LS-B11011-50

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Overview

Description
Huntingtin antibody LS-B11011 is an unconjugated rabbit polyclonal antibody to Huntingtin (HTT) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Huntingtin | Huntington disease protein | IT15 | HD | HTT | HD protein
Recommended Dilution: ELISA
1:20000 - 1:60000
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
0.1 µg/ml
UniProt Number
P42858
SwissProt
P42858

Target

Target
HTT / Huntingtin
Antigen Species
Human
Specificity
This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
Epitope
This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
Gene Name
HTT

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Huntington disease protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 30% Glycerol
Concentration
1.1 mg/mL
Animal Free
No
Carrier-Free
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.