LSBio
Polyclonal Rabbit anti-Schistosoma japonicum GST / Glutathione S-Transferase Antibody (IHC, WB)

Anti-GST Antibody - Western Blot. Western Blot showing detection of recombinant GST protein (0.25 ug) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti-Schistosoma japonicum GST / Glutathione S-Transferase Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Helminth / Nematode
Format:Liquid
See all Schistosoma japonicum GST / Glutathione S-Transferase antibodies →
$444.00each
Catalog Number: LS-C154218-100

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Overview

Description
Glutathione S-Transferase antibody LS-C154218 is an unconjugated rabbit polyclonal antibody to schistosoma japonicum Glutathione S-Transferase (GST). Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Helm
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:60000
Recommended Dilution: IHC
1:100
Recommended Dilution: WB
1:1000

Target

Target
Schistosoma japonicum GST / Glutathione S-Transferase
Antigen Species
Helminth / Nematode
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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