Anti-GST Antibody - Western Blot. anti-GST polyclonal antibody in western blot shows detection of recombinant GST (indicated by band at ~28 kD). The SDS-PAGE contained approximately 0.2 ug of rGST loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was transferred to nitrocellulose and blocked with "Blocking Buffer for Fluorescent Western Blot" MB-070 in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2000 dilution in blocking reagent, overnight at 4C. For detection DyLight800 conjugated Donkey-a-Goat IgG (p/n was used at a 1:20000 dilution (in blocking reagent) for 30 min at 25? C. Fluorescent data was collected on a LICOR Odyssey instrument.