Western Blot showing detection of recombinant GST protein (0.25 µg) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and
GST / Glutathione S-Transferase Antibody (AP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Helminth / Nematode |
| Format: | Liquid |
$564.00each
Catalog Number: LS-C154285-100
Catalog Number: LS-C154285
For bulk orders or custom formulations:
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- Description
- Glutathione S-Transferase antibody LS-C154285 is an AP-conjugated rabbit polyclonal antibody to schistosoma japonicum Glutathione S-Transferase (GST). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Helm
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2500
Target
- Target
- Schistosoma japonicum GST / Glutathione S-Transferase
- Antigen Species
- Helminth / Nematode
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- The immunogen is full length GST isolated from Schistosoma japonicum.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Alkaline Phosphatase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
- Concentration
- 0.5 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C. Do not freeze.
- Recommended Storage Buffer
- 0.05 M Tris-Chloride|0.15 M NaCl