Western Blot showing detection of recombinant GST protein (0.25 µg) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and
GST / Glutathione S-Transferase Antibody (TR)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Helminth / Nematode |
| Format: | Lyophilized |
$555.00each
Catalog Number: LS-C154304-100
Catalog Number: LS-C154304
For bulk orders or custom formulations:
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- Description
- Glutathione S-Transferase antibody LS-C154304 is a TR-conjugated rabbit polyclonal antibody to schistosoma japonicum Glutathione S-Transferase (GST). Validated for ELISA, Flow, IF and WB.
- Application
- ELISA, FC, IF, WB
- Reactivity
- Helm
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:60000
- Recommended Dilution: WB
- 1:2000 - 1:10000
Target
- Target
- Schistosoma japonicum GST / Glutathione S-Transferase
- Antigen Species
- Helminth / Nematode
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- The immunogen is full length GST isolated from Schistosoma japonicum.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Texas Red
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl