ELISA results of purified Goat anti-GST Antibody Biotin Conjugated tested against purified GST. Each well was coated in duplicate with 1.0 µg of GST The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate
GST / Glutathione S-Transferase Antibody (Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Helminth / Nematode |
| Format: | Lyophilized |
$822.00each
Catalog Number: LS-C154199-1
Catalog Number: LS-C154199
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- Description
- Glutathione S-Transferase antibody LS-C154199 is a biotin-conjugated goat polyclonal antibody to schistosoma japonicum Glutathione S-Transferase (GST). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Helm
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
Target
- Target
- Schistosoma japonicum GST / Glutathione S-Transferase
- Antigen Species
- Helminth / Nematode
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Glutathione-S-Transferase [Schistosoma japonicum].
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl