Detection of Human CD45 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
CD45 / LCA Antibody (aa1254-1304)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C291034-10
Catalog Number: LS-C291034
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- Description
- LCA antibody LS-C291034 is an unconjugated rabbit polyclonal antibody to human LCA (CD45) (aa1254-1304). Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
- Recommended Dilution: IHC
- 1:1000 - 1:10000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P08575
- NCBI GenBank
- NM_002838 | NP_002829.3
- SwissProt
- P08575
Target
- Target
- Human CD45 / LCA
- Antigen Species
- Human
- Epitope
- Region between residue 1254 to 1304 of human CD45 Molecule using the numbering given in entry P08575.2 (GeneID 5788).
- Gene Name
- PTPRC
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 5788 corresponding to amino acids between 1254 and 1304 of UniprotID: P08575
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate