LSBio
IHC-plus™ CD45 / LCA Antibody (clone F10-89-4, FITC)

Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ CD45 / LCA Antibody (clone F10-89-4, FITC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
See all CD45 / LCA antibodies →
$383.00each
Catalog Number: LS-B5327-0.05

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Overview

Description
LCA antibody LS-B5327 is an FITC-conjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
Recommended Dilution: FC
1:1
Recommended Dilution: IHC-P
1:50
UniProt Number
P08575
NCBI GenBank
NM_002838 | NP_002829.3
SwissProt
P08575

Target

Target
CD45 / LCA
Antigen Species
Human
Specificity
Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes
Epitope
Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes.
Gene Name
PTPRC

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
F10-89-4
Origin Species
Human

Immunogen

Immunogen
Human T lymphocytes

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 1% BSA
Concentration
0.1 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.