LSBio
IHC-plus™ CD45 / LCA Antibody (clone F10-89-4)

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml

IHC-plus™ CD45 / LCA Antibody (clone F10-89-4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Human
Format:Liquid
See all CD45 / LCA antibodies →
$383.00each
Catalog Number: LS-B15759-0.05

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Overview

Description
LCA antibody LS-B15759 is an unconjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-F, IHC-P, IP
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
Recommended Dilution: FC
1:10 - 1:50
Recommended Dilution: IHC-F
1:500 - 1:1000
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P08575
NCBI GenBank
NM_002838 | NP_002829.3
SwissProt
P08575

Target

Target
CD45 / LCA
Antigen Species
Human
Specificity
Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes
Epitope
Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes
Gene Name
PTPRC

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
F10-89-4
Origin Species
Human

Immunogen

Immunogen
Human T lymphocytes

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.