LSBio
PathPlus™ CD45 / LCA Antibody (clone PTPRC/1783R)

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ CD45 / LCA Antibody (clone PTPRC/1783R)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Recombinant Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all CD45 / LCA antibodies →
$536.00each
Catalog Number: LS-B16294-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
LCA antibody LS-B16294 is an unconjugated rabbit recombinant monoclonal antibody to human LCA (CD45). Validated for Flow, IF, IHC and WB.
Application
FC, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
Recommended Dilution: FC
1 - 2 µg/10E6 cells
Recommended Dilution: IF/ICC
1 - 2 µg/ml
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1 - 2 µg/ml
UniProt Number
P08575
NCBI GenBank
NM_002838 | NP_002829.3
SwissProt
P08575

Target

Target
CD45 / LCA
Antigen Species
Human
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Epitope
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Gene Name
PTPRC

Antibody

Host Species
Rabbit
Clonality
Recombinant Monoclonal
Clone
PTPRC/1783R
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human CD45RB protein. Antigen Molecular Weight: 180-220 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.