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PathPlus™ CD45 / LCA Antibody (clone PTPRC/1783R + PTPRC/1975R)

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ CD45 / LCA Antibody (clone PTPRC/1783R + PTPRC/1975R)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Recombinant Monoclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all CD45 / LCA antibodies →
$536.00each
Catalog Number: LS-B16296-100

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Overview

Description
LCA antibody LS-B16296 is an unconjugated rabbit recombinant monoclonal antibody to human LCA (CD45). Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1 - 2 µg/ml
UniProt Number
P08575
NCBI GenBank
NM_002838 | NP_002829.3
SwissProt
P08575

Target

Target
CD45 / LCA
Antigen Species
Human
Specificity
Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa ) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Epitope
Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180 kDa ) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
Gene Name
PTPRC

Antibody

Host Species
Rabbit
Clonality
Recombinant Monoclonal
Clone
PTPRC/1783R + PTPRC/1975R
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human CD45 protein. Antigen Molecular Weight: 180-220 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 2 years.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.