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NOTCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOTCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NOTCH1 antibodies →
$725.00each
Catalog Number: LS-G101485-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOTCH1 (NM_017617), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Notch 1 | NOTCH1 | TAN1
Origin Species
Human
NCBI GenBank
NM_017617
SwissProt
P46531

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
272.58 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPLLAPLLC LALLPALAAR GPRCSQPGET CLNGGKCEAA NGTEACVCGG AFVGPRCQDP
61NPCLSTPCKN AGTCHVVDRR GVADYACSCA LGFSGPLCLT PLDNACLTNP CRNGGTCDLL
121TLTEYKCRCP PGWSGKSCQQ ADPCASNPCA NGGQCLPFEA SYICHCPPSF HGPTCRQDVN
181ECGQKPGLCR HGGTCHNEVG SYRCVCRATH TGPNCERPYV PCSPSPCQNG GTCRPTGDVT
241HECACLPGFT GQNCEENIDD CPGNNCKNGG ACVDGVNTYN CRCPPEWTGQ YCTEDVDECQ
301LMPNACQNGG TCHNTHGGYN CVCVNGWTGE DCSENIDDCA SAACFHGATC HDRVASFYCE
361CPHGRTGLLC HLNDACISNP CNEGSNCDTN PVNGKAICTC PSGYTGPACS QDVDECSLGA
421NPCEHAGKCI NTLGSFECQC LQGYTGPRCE IDVNECVSNP CQNDATCLDQ IGEFQCICMP
481GYEGVHCEVN TDECASSPCL HNGRCLDKIN EFQCECPTGF TGHLCQYDVD ECASTPCKNG
541AKCLDGPNTY TCVCTEGYTG THCEVDIDEC DPDPCHYGSC KDGVATFTCL CRPGYTGHHC
601ETNINECSSQ PCRHGGTCQD RDNAYLCFCL KGTTGPNCEI NLDDCASSPC DSGTCLDKID
661GYECACEPGY TGSMCNINID ECAGNPCHNG GTCEDGINGF TCRCPEGYHD PTCLSEVNEC
721NSNPCVHGAC RDSLNGYKCD CDPGWSGTNC DINNNECESN PCVNGGTCKD MTSGYVCTCR
781EGFSGPNCQT NINECASNPC LNQGTCIDDV AGYKCNCLLP YTGATCEVVL APCAPSPCRN
841GGECRQSEDY ESFSCVCPTG WQAGQTCEVD INECVLSPCR HGASCQNTHG GYRCHCQAGY
901SGRNCETDID DCRPNPCHNG GSCTDGINTA FCDCLPGFRG TFCEEDINEC ASDPCRNGAN
961CTDCVDSYTC TCPAGFSGIH CENNTPDCTE SSCFNGGTCV DGINSFTCLC PPGFTGSYCQ
1021HDVNECDSQP CLHGGTCQDG CGSYRCTCPQ GYTGPNCQNL VHWCDSSPCK NGGKCWQTHT
1081QYRCECPSGW TGLYCDVPSV SCEVAAQRQG VDVARLCQHG GLCVDAGNTH HCRCQAGYTG
1141SYCEDLVDEC SPSPCQNGAT CTDYLGGYSC KCVAGYHGVN CSEEIDECLS HPCQNGGTCL
1201DLPNTYKCSC PRGTQGVHCE INVDDCNPPV DPVSRSPKCF NNGTCVDQVG GYSCTCPPGF
1261VGERCEGDVN ECLSNPCDAR GTQNCVQRVN DFHCECRAGH TGRRCESVIN GCKGKPCKNG
1321GTCAVASNTA RGFICKCPAG FEGATCENDA RTCGSLRCLN GGTCISGPRS PTCLCLGPFT
1381GPECQFPASS PCLGGNPCYN QGTCEPTSES PFYRCLCPAK FNGLLCHILD YSFGGGAGRD
1441IPPPLIEEAC ELPECQEDAG NKVCSLQCNN HACGWDGGDC SLNFNDPWKN CTQSLQCWKY
1501FSDGHCDSQC NSAGCLFDGF DCQRAEGQCN PLYDQYCKDH FSDGHCDQGC NSAECEWDGL
1561DCAEHVPERL AAGTLVVVVL MPPEQLRNSS FHFLRELSRV LHTNVVFKRD AHGQQMIFPY
1621YGREEELRKH PIKRAAEGWA APDALLGQVK ASLLPGGSEG GRRRRELDPM DVRGSIVYLE
1681IDNRQCVQAS SQCFQSATDV AAFLGALASL GSLNIPYKIE AVQSETVEPP PPAQLHFMYV
1741AAAAFVLLFF VGCGVLLSRK RRRQHGQLWF PEGFKVSEAS KKKRREPLGE DSVGLKPLKN
1801ASDGALMDDN QNEWGDEDLE TKKFRFEEPV VLPDLDDQTD HRQWTQQHLD AADLRMSAMA
1861PTPPQGEVDA DCMDVNVRGP DGFTPLMIAS CSGGGLETGN SEEEEDAPAV ISDFIYQGAS
1921LHNQTDRTGE TALHLAARYS RSDAAKRLLE ASADANIQDN MGRTPLHAAV SADAQGVFQI
1981LIRNRATDLD ARMHDGTTPL ILAARLAVEG MLEDLINSHA DVNAVDDLGK SALHWAAAVN
2041NVDAAVVLLK NGANKDMQNN REETPLFLAA REGSYETAKV LLDHFANRDI TDHMDRLPRD
2101IAQERMHHDI VRLLDEYNLV RSPQLHGAPL GGTPTLSPPL CSPNGYLGSL KPGVQGKKVR
2161KPSSKGLACG SKEAKDLKAR RKKSQDGKGC LLDSSGMLSP VDSLESPHGY LSDVASPPLL
2221PSPFQQSPSV PLNHLPGMPD THLGIGHLNV AAKPEMAALG GGGRLAFETG PPRLSHLPVA
2281SGTSTVLGSS SGGALNFTVG GSTSLNGQCE WLSRLQSGMV PNQYNPLRGS VAPGPLSTQA
2341PSLQHGMVGP LHSSLAASAL SQMMSYQGLP STRLATQPHL VQTQQVQPQN LQMQQQNLQP
2401ANIQQQQSLQ PPPPPPQPHL GVSSAASGHL GRSFLSGEPS QADVQPLGPS SLAVHTILPQ
2461ESPALPTSLP SSLVPPVTAA QFLTPPSQHS YSSPVDNTPS HQLQVPEHPF LTPSPESPDQ
2521WSSSSPHSNV SDWSEGVSSP PTSMQSQIAR IPEAFK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

2556 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.