NOTCH1 Antibody
Catalog Number: LS-C349283
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NOTCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G90569-20
Catalog Number: LS-G90569
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NOTCH1 (NM_017617) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF, and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Notch 1 | NOTCH1 | TAN1
- Origin Species
- Human
- NCBI GenBank
- NM_017617
- SwissProt
- P46531
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 272.58 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPLLAPLLC LALLPALAAR GPRCSQPGET CLNGGKCEAA NGTEACVCGG AFVGPRCQDP61NPCLSTPCKN AGTCHVVDRR GVADYACSCA LGFSGPLCLT PLDNACLTNP CRNGGTCDLL121TLTEYKCRCP PGWSGKSCQQ ADPCASNPCA NGGQCLPFEA SYICHCPPSF HGPTCRQDVN181ECGQKPGLCR HGGTCHNEVG SYRCVCRATH TGPNCERPYV PCSPSPCQNG GTCRPTGDVT241HECACLPGFT GQNCEENIDD CPGNNCKNGG ACVDGVNTYN CRCPPEWTGQ YCTEDVDECQ301LMPNACQNGG TCHNTHGGYN CVCVNGWTGE DCSENIDDCA SAACFHGATC HDRVASFYCE361CPHGRTGLLC HLNDACISNP CNEGSNCDTN PVNGKAICTC PSGYTGPACS QDVDECSLGA421NPCEHAGKCI NTLGSFECQC LQGYTGPRCE IDVNECVSNP CQNDATCLDQ IGEFQCICMP481GYEGVHCEVN TDECASSPCL HNGRCLDKIN EFQCECPTGF TGHLCQYDVD ECASTPCKNG541AKCLDGPNTY TCVCTEGYTG THCEVDIDEC DPDPCHYGSC KDGVATFTCL CRPGYTGHHC601ETNINECSSQ PCRHGGTCQD RDNAYLCFCL KGTTGPNCEI NLDDCASSPC DSGTCLDKID661GYECACEPGY TGSMCNINID ECAGNPCHNG GTCEDGINGF TCRCPEGYHD PTCLSEVNEC721NSNPCVHGAC RDSLNGYKCD CDPGWSGTNC DINNNECESN PCVNGGTCKD MTSGYVCTCR781EGFSGPNCQT NINECASNPC LNQGTCIDDV AGYKCNCLLP YTGATCEVVL APCAPSPCRN841GGECRQSEDY ESFSCVCPTG WQAGQTCEVD INECVLSPCR HGASCQNTHG GYRCHCQAGY901SGRNCETDID DCRPNPCHNG GSCTDGINTA FCDCLPGFRG TFCEEDINEC ASDPCRNGAN961CTDCVDSYTC TCPAGFSGIH CENNTPDCTE SSCFNGGTCV DGINSFTCLC PPGFTGSYCQ1021HDVNECDSQP CLHGGTCQDG CGSYRCTCPQ GYTGPNCQNL VHWCDSSPCK NGGKCWQTHT1081QYRCECPSGW TGLYCDVPSV SCEVAAQRQG VDVARLCQHG GLCVDAGNTH HCRCQAGYTG1141SYCEDLVDEC SPSPCQNGAT CTDYLGGYSC KCVAGYHGVN CSEEIDECLS HPCQNGGTCL1201DLPNTYKCSC PRGTQGVHCE INVDDCNPPV DPVSRSPKCF NNGTCVDQVG GYSCTCPPGF1261VGERCEGDVN ECLSNPCDAR GTQNCVQRVN DFHCECRAGH TGRRCESVIN GCKGKPCKNG1321GTCAVASNTA RGFICKCPAG FEGATCENDA RTCGSLRCLN GGTCISGPRS PTCLCLGPFT1381GPECQFPASS PCLGGNPCYN QGTCEPTSES PFYRCLCPAK FNGLLCHILD YSFGGGAGRD1441IPPPLIEEAC ELPECQEDAG NKVCSLQCNN HACGWDGGDC SLNFNDPWKN CTQSLQCWKY1501FSDGHCDSQC NSAGCLFDGF DCQRAEGQCN PLYDQYCKDH FSDGHCDQGC NSAECEWDGL1561DCAEHVPERL AAGTLVVVVL MPPEQLRNSS FHFLRELSRV LHTNVVFKRD AHGQQMIFPY1621YGREEELRKH PIKRAAEGWA APDALLGQVK ASLLPGGSEG GRRRRELDPM DVRGSIVYLE1681IDNRQCVQAS SQCFQSATDV AAFLGALASL GSLNIPYKIE AVQSETVEPP PPAQLHFMYV1741AAAAFVLLFF VGCGVLLSRK RRRQHGQLWF PEGFKVSEAS KKKRREPLGE DSVGLKPLKN1801ASDGALMDDN QNEWGDEDLE TKKFRFEEPV VLPDLDDQTD HRQWTQQHLD AADLRMSAMA1861PTPPQGEVDA DCMDVNVRGP DGFTPLMIAS CSGGGLETGN SEEEEDAPAV ISDFIYQGAS1921LHNQTDRTGE TALHLAARYS RSDAAKRLLE ASADANIQDN MGRTPLHAAV SADAQGVFQI1981LIRNRATDLD ARMHDGTTPL ILAARLAVEG MLEDLINSHA DVNAVDDLGK SALHWAAAVN2041NVDAAVVLLK NGANKDMQNN REETPLFLAA REGSYETAKV LLDHFANRDI TDHMDRLPRD2101IAQERMHHDI VRLLDEYNLV RSPQLHGAPL GGTPTLSPPL CSPNGYLGSL KPGVQGKKVR2161KPSSKGLACG SKEAKDLKAR RKKSQDGKGC LLDSSGMLSP VDSLESPHGY LSDVASPPLL2221PSPFQQSPSV PLNHLPGMPD THLGIGHLNV AAKPEMAALG GGGRLAFETG PPRLSHLPVA2281SGTSTVLGSS SGGALNFTVG GSTSLNGQCE WLSRLQSGMV PNQYNPLRGS VAPGPLSTQA2341PSLQHGMVGP LHSSLAASAL SQMMSYQGLP STRLATQPHL VQTQQVQPQN LQMQQQNLQP2401ANIQQQQSLQ PPPPPPQPHL GVSSAASGHL GRSFLSGEPS QADVQPLGPS SLAVHTILPQ2461ESPALPTSLP SSLVPPVTAA QFLTPPSQHS YSSPVDNTPS HQLQVPEHPF LTPSPESPDQ2521WSSSSPHSNV SDWSEGVSSP PTSMQSQIAR IPEAFKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
2556 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
