NOTCH1 Antibody
Catalog Number: LS-C349283
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
PathPlus™ NOTCH1 Antibody (aa2480-2510)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B398-50
Catalog Number: LS-B398
For bulk orders or custom formulations:
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- Description
- NOTCH1 antibody LS-B398 is an unconjugated rabbit polyclonal antibody to human NOTCH1 (aa2480-2510). Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Notch 1 | NOTCH1 | TAN1
- UniProt Number
- P46531
- NCBI GenBank
- NM_017617 | NP_060087.3
- SwissProt
- P46531
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:20000 - 1:100000 |
| IHC-P | 1:500 |
| WB | 1:2000 - 1:10000 |
Target
- Target
- NOTCH1
- Antigen Species
- Human
- Specificity
- No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
- Epitope
- No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
- Gene Name
- NOTCH1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 75 mg/mL
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006;66:1517-25. PubMed: 16452208
