LSBio
PathPlus™ NOTCH1 Antibody (aa2480-2510)

Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

PathPlus™ NOTCH1 Antibody (aa2480-2510)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B398-50

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Overview

Description
NOTCH1 antibody LS-B398 is an unconjugated rabbit polyclonal antibody to human NOTCH1 (aa2480-2510). Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Notch 1 | NOTCH1 | TAN1
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
P46531
NCBI GenBank
NM_017617 | NP_060087.3
SwissProt
P46531

Target

Target
NOTCH1
Antigen Species
Human
Specificity
No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
Epitope
No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
Gene Name
NOTCH1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
75 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006;66:1517-25. PubMed: 16452208
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