LSBio
NOTCH1 Antibody (aa2487-2501)

NOTCH1 Antibody (aa2487-2501)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human NOTCH1 antibodies →
$647.00each
Catalog Number: LS-C16928-100

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Overview

Description
NOTCH1 antibody LS-C16928 is an unconjugated rabbit polyclonal antibody to human NOTCH1 (aa2487-2501). Validated for ELISA, IHC and WB. Cited in 2 publications.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Notch 1 | NOTCH1 | TAN1
UniProt Number
P46531
SwissProt
P46531
Recommended Dilution
ApplicationDilution
ELISA1:10000 - 1:50000
IHC1:500 - 1:2500
WB1:1000 - 1:5000

Target

Target
Human NOTCH1
Antigen Species
Human
Epitope
Recognizes human NOTCH 1. No cross-reactivity with NOTCH 2 or Mouse Notch.
Gene Name
NOTCH1

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide C-QHSYSSPVDNTPSHQ corresponding to amino acid residues 2488-2502 of human Notch 1, intracellular domain. A residue of cysteine was added to the N-Terminus end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla (100%); Opossum, Chicken, Xenopus, Newt (93%); Platypus (87%); Trout, Stickleback, Pufferfish, Zebrafish (80%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.1% sodium azide. No stabilizing proteins added.
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum. Rapacioli M, Botelho J, Cerda G, Duarte S, Elliot M, Palma V, Flores V. BMC neuroscience. 2012;13:117.

PubMed: 23031710 · PMC: PMC3564940


Temporal-spatial correlation between angiogenesis and corticogenesis in the developing chick optic tectum. Alejandra Rodriguez Celin, Melina Rapacioli, Mariela Azul Gonzalez, Virginia Laura Ballarin, Sara Fiszer de Plazas, Juan José López-Costa, Vladimir Flores. PloS one. 2015 January;10:e0116343.

PubMed: 25633659 · PMC: PMC4310613

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