Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MYOCD / Myocardin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128823-100
Catalog Number: LS-G128823
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Myocardin(MYOCD) (NM_001146312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MYOCD | MYCD | Myocardin
- Origin Species
- Human
- NCBI GenBank
- NM_153604
- SwissProt
- Q8IZQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLLGSEHSL LIRSKFRSVL QLRLQQRRTQ EQLANQGIIP PLKRPAEFHE QRKHLDSDKA61KNSLKRKARN RCNSADLVNM HILQASTAER SIPTAQMKLK RARLADDLNE KIALRPGPLE121LVEKNILPVD SAVKEAIKGN QVSFSKSTDA FAFEEDSSSD GLSPDQTRSE DPQNSAGSPP181DAKASDTPST GSLGTNQDLA SGSENDRNDS ASQPSHQSDA GKQGLGPPST PIAVHAAVKS241KSLGDSKNRH KKPKDPKPKV KKLKYHQYIP PDQKAEKSPP PMDSAYARLL QQQQLFLQLQ301ILSQQQQQQQ HRFSYLGMHQ AQLKEPNEQM VRNPNSSSTP LSNTPLSPVK NSFSGQTGVS361SFKPGPLPPN LDDLKVSELR QQLRIRGLPV SGTKTALMDR LRPFQDCSGN PVPNFGDITT421VTFPVTPNTL PNYQSSSSTS ALSNGFYHFG STSSSPPISP ASSDLSVAGS LPDTFNDASP481SFGLHPSPVH VCTEESLMSS LNGGSVPSEL DGLDSEKDKM LVEKQKVINE LTWKLQQEQR541QVEELRMQLQ KQKRNNCSEK KPLPFLAASI KQEEAVSSCP FASQVPVKRQ SSSSECHPPA601CEAAQLQPLG NAHCVESSDQ TNVLSSTFLS PQCSPQHSPL GAVKSPQHIS LPPSPNNPHF661LPSSSGAQGE GHRVSSPISS QVCTAQNSGA HDGHPPSFSP HSSSLHPPFS GAQADSSHGA721GGNPCPKSPC VQQKMAGLHS SDKVGPKFSI PSPTFSKSSS AISEVTQPPS YEDAVKQQMT781RSQQMDELLD VLIESGEMPA DAREDHSCLQ KVPKIPRSSR SPTAVLTKPS ASFEQASSGS841QIPFDPYATD SDEHLEVLLN SQSPLGKMSD VTLLKIGSEE PHFDGIMDGF SGKAAEDLFN901AHEILPGPLS PMQTQFSPSS VDSNGLQLSF TESPWETMEW LDLTPPNSTP GFSALTTSSP961SIFNIDFLDV TDLNLNSSMD LHLQQWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
986 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.