Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MYOCD / Myocardin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128312-100
Catalog Number: LS-G128312
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Myocardin(MYOCD) (NM_001146313), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MYOCD | MYCD | Myocardin
- Origin Species
- Human
- NCBI GenBank
- NM_153604
- SwissProt
- Q8IZQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLKRARLAD DLNEKIALRP GPLELVEKNI LPVDSAVKEA IKGNQVSFSK STDAFAFEED61SSSDGLSPDQ TRSEDPQNSA GSPPDAKASD TPSTGSLGTN QDLASGSEND RNDSASQPSH121QSDAGKQGLG PPSTPIAVHA AVKSKSLGDS KNRHKKPKDP KPKVKKLKYH QYIPPDQKAE181KSPPPMDSAY ARLLQQQQLF LQLQILSQQQ QQQQHRFSYL GMHQAQLKEP NEQMVRNPNS241SSTPLSNTPL SPVKNSFSGQ TGVSSFKPGP LPPNLDDLKV SELRQQLRIR GLPVSGTKTA301LMDRLRPFQD CSGNPVPNFG DITTVTFPVT PNTLPNYQSS SSTSALSNGF YHFGSTSSSP361PISPASSDLS VAGSLPDTFN DASPSFGLHP SPVHVCTEES LMSSLNGGSV PSELDGLDSE421KDKMLVEKQK VINELTWKLQ QEQRQVEELR MQLQKQKRNN CSEKKPLPFL AASIKQEEAV481SSCPFASQVP VKRQSSSSEC HPPACEAAQL QPLGNAHCVE SSDQTNVLSS TFLSPQCSPQ541HSPLGAVKSP QHISLPPSPN NPHFLPSSSG AQGEGHRVSS PISSQVCTAQ NSGAHDGHPP601SFSPHSSSLH PPFSGAQADS SHGAGGNPCP KSPCVQQKMA GLHSSDKVGP KFSIPSPTFS661KSSSAISEVT QPPSYEDAVK QVTMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
684 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.