LSBio
Human MYOCD / Myocardin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MYOCD / Myocardin Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MYOCD antibodies →
$220.00each
Catalog Number: LS-G93069-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Myocardin(MYOCD) (NM_001146313) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYOCD | MYCD | Myocardin
Origin Species
Human
NCBI GenBank
NM_153604
SwissProt
Q8IZQ8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKLKRARLAD DLNEKIALRP GPLELVEKNI LPVDSAVKEA IKGNQVSFSK STDAFAFEED
61SSSDGLSPDQ TRSEDPQNSA GSPPDAKASD TPSTGSLGTN QDLASGSEND RNDSASQPSH
121QSDAGKQGLG PPSTPIAVHA AVKSKSLGDS KNRHKKPKDP KPKVKKLKYH QYIPPDQKAE
181KSPPPMDSAY ARLLQQQQLF LQLQILSQQQ QQQQHRFSYL GMHQAQLKEP NEQMVRNPNS
241SSTPLSNTPL SPVKNSFSGQ TGVSSFKPGP LPPNLDDLKV SELRQQLRIR GLPVSGTKTA
301LMDRLRPFQD CSGNPVPNFG DITTVTFPVT PNTLPNYQSS SSTSALSNGF YHFGSTSSSP
361PISPASSDLS VAGSLPDTFN DASPSFGLHP SPVHVCTEES LMSSLNGGSV PSELDGLDSE
421KDKMLVEKQK VINELTWKLQ QEQRQVEELR MQLQKQKRNN CSEKKPLPFL AASIKQEEAV
481SSCPFASQVP VKRQSSSSEC HPPACEAAQL QPLGNAHCVE SSDQTNVLSS TFLSPQCSPQ
541HSPLGAVKSP QHISLPPSPN NPHFLPSSSG AQGEGHRVSS PISSQVCTAQ NSGAHDGHPP
601SFSPHSSSLH PPFSGAQADS SHGAGGNPCP KSPCVQQKMA GLHSSDKVGP KFSIPSPTFS
661KSSSAISEVT QPPSYEDAVK QVTM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.