MYOCD / Myocardin Antibody
Catalog Number: LS-C137255-100
100 µL (1 mg/mL) / $339.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MYOCD / Myocardin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G93071-20
Catalog Number: LS-G93071
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Myocardin(MYOCD) (NM_001146312) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MYOCD | MYCD | Myocardin
- Origin Species
- Human
- NCBI GenBank
- NM_153604
- SwissProt
- Q8IZQ8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLLGSEHSL LIRSKFRSVL QLRLQQRRTQ EQLANQGIIP PLKRPAEFHE QRKHLDSDKA61KNSLKRKARN RCNSADLVNM HILQASTAER SIPTAQMKLK RARLADDLNE KIALRPGPLE121LVEKNILPVD SAVKEAIKGN QVSFSKSTDA FAFEEDSSSD GLSPDQTRSE DPQNSAGSPP181DAKASDTPST GSLGTNQDLA SGSENDRNDS ASQPSHQSDA GKQGLGPPST PIAVHAAVKS241KSLGDSKNRH KKPKDPKPKV KKLKYHQYIP PDQKAEKSPP PMDSAYARLL QQQQLFLQLQ301ILSQQQQQQQ HRFSYLGMHQ AQLKEPNEQM VRNPNSSSTP LSNTPLSPVK NSFSGQTGVS361SFKPGPLPPN LDDLKVSELR QQLRIRGLPV SGTKTALMDR LRPFQDCSGN PVPNFGDITT421VTFPVTPNTL PNYQSSSSTS ALSNGFYHFG STSSSPPISP ASSDLSVAGS LPDTFNDASP481SFGLHPSPVH VCTEESLMSS LNGGSVPSEL DGLDSEKDKM LVEKQKVINE LTWKLQQEQR541QVEELRMQLQ KQKRNNCSEK KPLPFLAASI KQEEAVSSCP FASQVPVKRQ SSSSECHPPA601CEAAQLQPLG NAHCVESSDQ TNVLSSTFLS PQCSPQHSPL GAVKSPQHIS LPPSPNNPHF661LPSSSGAQGE GHRVSSPISS QVCTAQNSGA HDGHPPSFSP HSSSLHPPFS GAQADSSHGA721GGNPCPKSPC VQQKMAGLHS SDKVGPKFSI PSPTFSKSSS AISEVTQPPS YEDAVKQQMT781RSQQMDELLD VLIESGEMPA DAREDHSCLQ KVPKIPRSSR SPTAVLTKPS ASFEQASSGS841QIPFDPYATD SDEHLEVLLN SQSPLGKMSD VTLLKIGSEE PHFDGIMDGF SGKAAEDLFN901AHEILPGPLS PMQTQFSPSS VDSNGLQLSF TESPWETMEW LDLTPPNSTP GFSALTTSSP961SIFNIDFLDV TDLNLNSSMD LHLQQWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
986 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
