WDR25 Antibody (aa444-473)
Catalog Number: LS-C157364-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human WDR25 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82950-20
Catalog Number: LS-G82950
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WDR25 (NM_024515) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf67 | WD repeat domain 25 | WDR25
- Origin Species
- Human
- NCBI GenBank
- NM_024515
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTARTLSLMA SLVAYDDSDS EAETEHAGSF NATGQQKDTS GVARPPGQDF ASGTLDVPKA61GAQPTKHGSC EDPGGYRLPL AQLGRSDWGS CPSQRLQWPG KEPQVTFPIK EPSCSSLWTS121HVPASHMPLA AARFKQVKLS RNFPKSSFHA QSESETVGKN GSSFQKKKCE DCVVPYTPRR181LRQRQALSTE TGKGKDVEPQ GPPAGRAPAP LYVGPGVSEF IQPYLNSHYK ETTVPRKVLF241HLRGHRGPVN TIQWCPVLSK SHMLLSTSMD KTFKVWNAVD SGHCLQTYSL HTEAVRAARW301APCGRRILSG GFDFALHLTD LETGTQLFSG RSDFRITTLK FHPKDHNIFL CGGFSSEMKA361WDIRTGKVMR SYKATIQQTL DILFLREGSE FLSSTDASTR DSADRTIIAW DFRTSAKISN421QIFHERFTCP SLALHPREPV FLAQTNGNYL ALFSTVWPYR MSRRRRYEGH KVEGYSVGCE481CSPGGDLLVT GSADGRVLMY SFRTASRACT LQGHTQACVG TTYHPVLPSV LATCSWGGDM541KIWHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
544 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
