Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WDR23 / DCAF11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104498-100
Catalog Number: LS-G104498
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR23(DCAF11) (NM_181357), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DCAF11 | WD repeat domain 23 | WDR23 | GL014 | PRO2389
- Origin Species
- Human
- NCBI GenBank
- NM_025230
- SwissProt
- Q8TEB1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSRNSSSAG SGSGDPSEGL PRRGAGLRRS EEEEEEDEDV DLAQALLDSE EENDRAWDGR61LGDRYNPPVD ATPDTRELEF NEIKTQVELA TGQLGLRRAA QKHSFPRMLH QRERGLCHRG121SFSLGEQSRV ISHFLPNDLG FTDSYSQKAF CGIYSKDGQI FMSACQDQTI RLYDCRYGRF181RKFKSIKARD VGWSVLDVAF TPDGNHFLYS SWSDYIHICN IYGEGDTHTA LDLRPDERRF241AVFSIAVSSD GREVLGGAND GCLYVFDREQ NRRTLQIESH EDDVNAVAFA DISSQILFSG301GDDAICKVWD RRTMREDDPK PVGALAGHQD GITFIDSKGD ARYLISNSKD QTIKLWDIRR361FSSREGMEAS RQAATQQNWD YRWQQVPKKA WRKLKLPGDS SLMTYRGHGV LHTLIRCRFS421PIHSTGQQFI YSGCSTGKVV VYDLLSGHIV KKLTNHKACV RDVSWHPFEE KIVSSSWDGN481LRLWQYRQAE YFQDDMPESE ECASAPAPVP QSSTPFSSPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.