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WDR20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR20 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR20 antibodies →
$725.00each
Catalog Number: LS-G104563-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR20 (NM_181291), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DMR | WD repeat domain 20 | Protein DMR | WDR20
Origin Species
Human
SwissProt
Q8TBZ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATEGGGKEM NEIKTQFTTR EGLYKLLPHS EYSRPNRVPF NSQGSNPVRV SFVNLNDQSG
61NGDRLCFNVG RELYFYIYKG VRKAADLSKP IDKRIYKGTQ PTCHDFNHLT ATAESVSLLV
121GFSAGQVQLI DPIKKETSKL FNEERLIDKS RVTCVKWVPG SESLFLVAHS SGNMYLYNVE
181HTCGTTAPHY QLLKQGESFA VHTCKSKSTR NPLLKWTVGE GALNEFAFSP DGKFLACVSQ
241DGFLRVFNFD SVELHGTMKS YFGGLLCVCW SPDGKYIVTG GEDDLVTVWS FVDCRVIARG
301HGHKSWVSVV AFDPYTTSVE EGDPMEFSGS DEDFQDLLHF GRDRANSTQS RLSKRNSTDS
361RPVSVTYRFG SVGQDTQLCL WDLTEDILFP HQPLSRARTH TNVMNATSPP AGSNGNSVTT
421PGNSVPPPLP RSNSLPHSAV SNAGSKSSVM DGAIASGVSK FATLSLHDRK ERHHEKDHKR
481NHSMGHISSK SSDKLNLVTK TKTDPAKTLG TPLCPRMEDV PLLEPLICKK IAHERLTVLI
541FLEDCIVTAC QEGFICTWGR PGKVGSLSSP SQASSPGGTV V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.