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WDR25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR25 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR25 antibodies →
$725.00each
Catalog Number: LS-G109949-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR25 (NM_024515), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf67 | WD repeat domain 25 | WDR25
Origin Species
Human
NCBI GenBank
NM_024515

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MTARTLSLMA SLVAYDDSDS EAETEHAGSF NATGQQKDTS GVARPPGQDF ASGTLDVPKA
61GAQPTKHGSC EDPGGYRLPL AQLGRSDWGS CPSQRLQWPG KEPQVTFPIK EPSCSSLWTS
121HVPASHMPLA AARFKQVKLS RNFPKSSFHA QSESETVGKN GSSFQKKKCE DCVVPYTPRR
181LRQRQALSTE TGKGKDVEPQ GPPAGRAPAP LYVGPGVSEF IQPYLNSHYK ETTVPRKVLF
241HLRGHRGPVN TIQWCPVLSK SHMLLSTSMD KTFKVWNAVD SGHCLQTYSL HTEAVRAARW
301APCGRRILSG GFDFALHLTD LETGTQLFSG RSDFRITTLK FHPKDHNIFL CGGFSSEMKA
361WDIRTGKVMR SYKATIQQTL DILFLREGSE FLSSTDASTR DSADRTIIAW DFRTSAKISN
421QIFHERFTCP SLALHPREPV FLAQTNGNYL ALFSTVWPYR MSRRRRYEGH KVEGYSVGCE
481CSPGGDLLVT GSADGRVLMY SFRTASRACT LQGHTQACVG TTYHPVLPSV LATCSWGGDM
541KIWH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.