ELF1 Antibody
Catalog Number: LS-C416124
2 sizes / from $312.00
Antibody: Rabbit Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ELF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G75834-20
Catalog Number: LS-G75834
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human E74 like factor 1(ELF1) (NM_001145353) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ELF1 | E74-like factor 1
- Origin Species
- Human
- NCBI GenBank
- NM_172373
- SwissProt
- P32519
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM61ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI121NGIPEVMETQ QVQEKYADSP GASSPEQPKR KKGRKTKPPR PDSPATTPNI SVKKKNKDGK181GNTIYLWEFL LALLQDKATC PKYIKWTQRE KGIFKLVDSK AVSRLWGKHK NKPDMNYETM241GRALRYYYQR GILAKVEGQR LVYQFKEMPK DLIYINDEDP SSSIESSDPS LSSSATSNRN301QTSRSRVSSS PGVKGGATTV LKPGNSKAAK PKDPVEVAQP SEVLRTVQPT QSPYPTQLFR361TVHVVQPVQA VPEGEAARTS TMQDETLNSS VQSIRTIQAP TQVPVVVSPR NQQLHTVTLQ421TVPLTTVIAS TDPSAGTGSQ KFILQAIPSS QPMTVLKENV MLQSQKAGSP PSIVLGPAQV481QQVLTSNVQT ICNGTVSVAS SPSFSATAPV VTFSPRSSQL VAHPPGTVIT SVIKTQETKT541LTQEVEKKES EDHLKENTEK TEQQPQPYVM VVSSSNGFTS QVAMKQNELL EPNSFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
595 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
