LSBio
ELF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ELF1 antibodies →
$504.00each
Catalog Number: LS-G126666-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human E74 like factor 1(ELF1) (NM_001145353), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ELF1 | E74-like factor 1
Origin Species
Human
NCBI GenBank
NM_172373
SwissProt
P32519

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVVQQNDL VFEFASNVME DERQLGDPAI FPAVIVEHVP GADILNSYAG LACVEEPNDM
61ITESSLDVAE EEIIDDDDDD ITLTVEASCH DGDETIETIE AAEALLNMDS PGPMLDEKRI
121NGIPEVMETQ QVQEKYADSP GASSPEQPKR KKGRKTKPPR PDSPATTPNI SVKKKNKDGK
181GNTIYLWEFL LALLQDKATC PKYIKWTQRE KGIFKLVDSK AVSRLWGKHK NKPDMNYETM
241GRALRYYYQR GILAKVEGQR LVYQFKEMPK DLIYINDEDP SSSIESSDPS LSSSATSNRN
301QTSRSRVSSS PGVKGGATTV LKPGNSKAAK PKDPVEVAQP SEVLRTVQPT QSPYPTQLFR
361TVHVVQPVQA VPEGEAARTS TMQDETLNSS VQSIRTIQAP TQVPVVVSPR NQQLHTVTLQ
421TVPLTTVIAS TDPSAGTGSQ KFILQAIPSS QPMTVLKENV MLQSQKAGSP PSIVLGPAQV
481QQVLTSNVQT ICNGTVSVAS SPSFSATAPV VTFSPRSSQL VAHPPGTVIT SVIKTQETKT
541LTQEVEKKES EDHLKENTEK TEQQPQPYVM VVSSSNGFTS QVAMKQNELL EPNSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

595 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.