LSBio
Human CCDC178 / C18orf34 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CCDC178 / C18orf34 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CCDC178 antibodies →
$220.00each
Catalog Number: LS-G96176-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C18orf34(CCDC178) (NM_198995) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001105528

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTENKTVSSS STRDDQTNIG LTCQEVKALR EKAWSRTNEG NAMSQSLVLY GASKENSEGF
61HESKMTNTEG VNKGIYFSYP CRRHSCAVVN IPAPCVNKMI SHIQDVESKI QEHLKRFETS
121FEEWSRTSST KDLKEDWSVT TPVKEVKPGE KRDEKCPELK QEMETLLSEA IRLIKSLETD
181RADAEEALKQ QRSRKNMINM KIDSWSVWKL QELPLAVQKE HEAYLSDVIE LQWHLEDKAN
241QLQHFEKQKT ELEEANAKIQ ADIDYMNEHG PLLDSKQNQE LQDLKNHYKK KMEVMDLHRK
301VNEELEEALE ACENARLKAQ QIKEEIDKDI YQDEKTIEAY KREIYQLNSL FDHYSSSVIN
361VNTNIEEKEE EVTEAIRETK SSKNELHSLS KMLEDLRRVY DQLTWKQKSH ENQYLEAVND
421FYAAKKTWDI ELSDVAKDFS AISLACTKLT EDNKKLEIDI NKITVKTNES IRKKSKYESE
481IKYLTIMKLK NDKHLKNIYK EAYRIGTLFH LTKHKTDEME DKIAEVRRKF KGREEFLKKL
541TQGEVAAGMV LQKKLYSIYE VQALERKELI KNRAICAMSL AELQEPLLQL EDEAERIRSL
601DKEHSVSKRS AIFKDLEATK SKTMIFYAKI NELNEELKAK EEEKKSFDQT LEILKNKFIT
661MRFKREHAQT VFDHYMQEKK DCEERIFEED QRFRVLLAVR QKTLQDTQKI IADSLEENLR
721LAQEYQQLQI TFLKEKDNYF NIYDKQLSLD TSIRDKKQLC QLQRRMHTLW QEHFKLVVLF
781SQMRLANFQT DSQESIQKIL AVQEESSNLM QHILGFFQTL TDGTCENDG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

829 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.