Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCDC178 / C18orf34 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103477-100
Catalog Number: LS-G103477
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C18orf34(CCDC178) (NM_198995), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001105528
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 97.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTENKTVSSS STRDDQTNIG LTCQEVKALR EKAWSRTNEG NAMSQSLVLY GASKENSEGF61HESKMTNTEG VNKGIYFSYP CRRHSCAVVN IPAPCVNKMI SHIQDVESKI QEHLKRFETS121FEEWSRTSST KDLKEDWSVT TPVKEVKPGE KRDEKCPELK QEMETLLSEA IRLIKSLETD181RADAEEALKQ QRSRKNMINM KIDSWSVWKL QELPLAVQKE HEAYLSDVIE LQWHLEDKAN241QLQHFEKQKT ELEEANAKIQ ADIDYMNEHG PLLDSKQNQE LQDLKNHYKK KMEVMDLHRK301VNEELEEALE ACENARLKAQ QIKEEIDKDI YQDEKTIEAY KREIYQLNSL FDHYSSSVIN361VNTNIEEKEE EVTEAIRETK SSKNELHSLS KMLEDLRRVY DQLTWKQKSH ENQYLEAVND421FYAAKKTWDI ELSDVAKDFS AISLACTKLT EDNKKLEIDI NKITVKTNES IRKKSKYESE481IKYLTIMKLK NDKHLKNIYK EAYRIGTLFH LTKHKTDEME DKIAEVRRKF KGREEFLKKL541TQGEVAAGMV LQKKLYSIYE VQALERKELI KNRAICAMSL AELQEPLLQL EDEAERIRSL601DKEHSVSKRS AIFKDLEATK SKTMIFYAKI NELNEELKAK EEEKKSFDQT LEILKNKFIT661MRFKREHAQT VFDHYMQEKK DCEERIFEED QRFRVLLAVR QKTLQDTQKI IADSLEENLR721LAQEYQQLQI TFLKEKDNYF NIYDKQLSLD TSIRDKKQLC QLQRRMHTLW QEHFKLVVLF781SQMRLANFQT DSQESIQKIL AVQEESSNLM QHILGFFQTL TDGTCENDGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
829 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.