LSBio
CCDC158 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC158 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC158 antibodies →
$725.00each
Catalog Number: LS-G119424-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC158 (NM_001042784), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC158
Origin Species
Human
NCBI GenBank
NM_001042784
SwissProt
Q5M9N0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
127 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESKAWESNN EDLLSSSGVT SNGGSSSSFF VSSIRGTIIE NTSSAGTLTQ VPFFPKYEVE
61LDSPRKIIPS PGKEHFERVL EEYSHQVKDL QRRLNESNEL HEKQKFYLRQ SVIDLQTKLQ
121EMQMERDAMA DIRRRESQSQ EDLRNQLQNT VHELEAAKCL KEDMLKDSNT QIEQLRKMML
181SHEGVLQEIR SILVDFEEAS GKKICEHDSM STLHFRSLGS AISKILRELD TEISYLKGRI
241FPVEDQLEAL KSESQNKIEL LLQQHQDRIE QLISEHEVEI TGLTEKASSA RSQANSIQSQ
301MEIIQEQARN QNSMYMRQLS DLESTVSQLR SELREAKRMY EDKTEELEKQ LVLANSELTE
361ARTERDQFSQ ESGNLDDQLQ KLLADLHKRE KELSLEKEQN KRLWDRDTGN SITIDHLRRE
421LDNRNMEVQR LEALLKALKS ECQGQMERQM AAIQGKNESL EKVSSLTAQL ESTKEMLRKV
481VEELTAKKMT LESSERTISD LTTSLQEKER AIEATNAEIT KLRSRVDLKL QELQHLKNEG
541DHLRNVQTEC EALKLQMTEK DKVIEILRQQ IENMTQLVGQ HGRTAGAMQV EKAQLEKEIN
601DRRMELKELK ILKDKKDAKI RELEARVSDL ELEKVKLVNA GSERLRAVKD IKQERDQLLN
661EVKTSRSELN NLSEEYEVLK RNFRNKSEEM EMTTNKLKMQ LKSAQSELEQ TRNTLKSMEG
721SDGHAMKVAM GMQKQITAKR GQIDALQSKI QFLEEAMTNA NKEKHFLKEE KSKLSQELST
781VATEKNKMAG ELEVLRSQER RLKEKVTNME VALDKASLQF AECQDIIQRQ EQESVRLKLQ
841HTLDIKELQG PGYTSNSSLK PRLLQPASVT RSHSNVPSSQ STASFLSHHS TKANTLKEDP
901TRDLKQLLQE LRSVINEEPA VSLSKTEEDG RTSLGALEDR VRDCITESSL RSDMCHRSNN
961SLRDSTEGSK SSETLSREPV TLHAGDREDP SGCFTFTSAA SPSVKNSASR SFNSSPKKSP
1021VHSLLTSSVE GSIGSTSQYR SAKPIHSSDS VKDSQSPPIE TTGKTCRKLQ NRLESLQTLV
1081EDLQLKNQAM SSMIRNQEKR IQKVKDQEKM LLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1113 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.